Speaker
Mr
Jae-ik Shin
(National Cancer Center at Korea)
Description
Objective
In order to evaluate space mission hazards, we need to understand the effects of ionizing radiation in the human body on a microscopic scale. DNA damages impact directly on the fate of irradiated biological cells. Early DNA damages result from physical, physico-chemical and chemical interactions on cellular DNA. In this study, we present the experimental irradiation of plasmid DNA using protons. DNA damages have been quantified using electrophoresis. Simulation results using the Geant4-DNA extension of the general purpose Geant4 Monte Carlo toolkit are also presented.
Methods
The National Cancer Center (NCC) is equipped with a proton therapy cyclotron facility including the IBA Beam Nozzle. We used a proton beam Bragg peak of range 14 cm. The setup has been fully simulated with Geant4 and the validation of proton scattering is currently on going. Thanks to these simulations, it is now possible to simulate the characteristics of the beam at nozzle exit.
Plasmid DNA samples were exposed to the proton beam at nozzle exit and DNA damages were measured indirectly using electrophoresis.
Results
We analysed dose distribution in matter using Geant4. Experimental data were compared with simulation results. In these results, we found the relationship between DNA damages and dose. Further validation is needed using more biological experiments
Primary author
Mr
Jae-ik Shin
(National Cancer Center at Korea)
Co-authors
Dr
Jungwook Shin
(UCSF Medical Center, USA)
Dr
Se Bbyeong Lee
(National Cancer Center, Republic of Korea)
Dr
Sebastien Incerti
(Centre d Etudes Nucleaires de Bordeaux-Gradig, Universite Bordeaux 1)
Mr
Seyjoon Park
(National Cancer Center, Republic of Korea)
